The Essay Writing Samples Easy Game

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Gram Stain Procedure in Microbiology

The Gram stain is a differential method of staining used to assign bacteria to one of two groups (gram-positive and gram-negative) based on the properties of their cell walls. It is also known as Gram staining or Grams method. The procedure is named for the person who developed the technique, Danish bacteriologist Hans Christian Gram. How the Gram Stain Works The procedure is based on the reaction between peptidoglycan in the cell walls of some bacteria. The Gram stain involves staining bacteria, fixing the color with a mordant, decolorizing the cells, and applying a counterstain. The primary stain (crystal violet) binds to peptidoglycan, coloring cells purple. Both gram-positive and gram-negative cells have peptidoglycan in their cell walls, so initially, all bacteria stain violet.Grams iodine (iodine and potassium iodide) is applied as a mordant or fixative. Gram-positive cells form a crystal violet-iodine complex.Alcohol or acetone is used to decolorize the cells. Gram-negative bacteria have much less peptidoglycan in their cell walls, so this step essentially renders them colorless, while only some of the color is removed from gram-positive cells, which have more peptidoglycan (60-90% of the cell wall). The thick cell wall of gram-positive cells is dehydrated by the decolorizing step, causing them to shrink and trapping the stain-iodine complex inside.After the decolorizing step, a counterstain is applied (usually safranin, but sometimes fuchsine) to color the bacteria pink. Both gram-positive and gram-negative bacteria pick up the pink stain, but it is no t visible over the darker purple of the gram-positive bacteria. If the staining procedure is performed correctly, gram-positive bacteria will be purple, while gram-negative bacteria will be pink. Purpose of the Gram Staining Technique The results of the Gram stain are viewed using light microscopy. Because the bacteria are colored, not only is their Gram stain group identified, but their shape, size, and clumping pattern may be observed. This makes the Gram stain a valuable diagnostic tool for a medical clinic or lab. While the stain may not definitely identify bacteria, often knowing whether they are gram-positive or gram-negative is sufficient for prescribing an effective antibiotic. Limitations of the Technique Some bacteria may be gram-variable or gram-indeterminate. However, even this information may be useful in narrowing down bacterial identity. The technique is most reliable when cultures are less than 24 hours old. While it can be used on broth cultures, its best to centrifuge them first. The primary limitation of the technique is that it yields erroneous results if mistakes are made in the technique. Practice and skill are needed to produce a reliable result. Also, an infectious agent may not be bacterial. Eukaryotic pathogens stain gram-negative. However, most eukaryotic cells except fungi (including yeast) fail to stick to the slide during the process. Gram Staining Procedure Materials Crystal violet (primary stain)Grams iodine (mordant, to fix crystal violet in the cell wall)Ethanol or Acetone (decolorizer)Safranin (secondary stain or counterstain)Water in a squirt bottle or dropper bottleMicroscope slidesCompound microscope Steps Place a small drop of bacterial sample on a slide. Heat fix the bacteria to the slide by passing it through the flame of a Bunsen burner three times. Applying too much heat or for too long can melt the bacteria  cell walls, distorting their shape and leading to an inaccurate result. If too little heat is applied, the bacteria will wash off the slide during staining.Use a dropper to apply the primary stain (crystal violet) to the slide and allow it to sit for 1 minute. Gently rinse the slide with water no longer than 5 seconds to remove excess stain. Rinsing too long can remove too much color, while not rinsing long enough may allow too much stain to remain on gram-negative cells.Use a dropper to apply Grams iodine to the slide to fix the crystal violet to the cell wall. Let it sit for 1 minute.Rinse the slide with alcohol or acetone about 3 seconds, followed immediately with a gentle rinse using water. The gram-negative cells will lose color, while the gram-positive cells will rema in violet or blue. However, if the decolorizer is left on too long, all cells will lose color!Apply the secondary stain, safranin, and allow it to sit for 1 minute. Gently rinse with water no longer than 5 seconds. The gram-negative cells should be stained red or pink, while the gram-positive cells will still appear purple or blue.View the slide using a compound microscope. A magnification of 500x to 1000x may be needed to distinguish cell shape and arrangement. Examples of Gram-Positive and Gram-Negative Pathogens Not all bacteria identified by the Gram stain are associated with diseases, but a few important examples include: Gram-positive cocci  (round): Staphylococcus aureusGram-negative cocci:  Neisseria meningitidisGram-positive bacilli (rods):  Bacillus anthracisGram-negative bacilli:  Escherichia coli

Domestic Violence in the United States Essay - 2145 Words

Student Name: Nathan Regedanz Project 1 SOCY 100 Date: 20 April 2009 Introduction The office of domestic violence against women defines domestic violence as pattern of abusive behavior in any relationship that is used by one partner to gain or maintain power and control over another intimate partner. Domestic violence does not just affect the immediate victim but is a widespread problem in the United States. Eighthly five percent of domestic violence victims are women (Domestic Violence Facts, 2007). Results of violence can be health care professionals in the treatment of physical injuries, the psychological impact upon the victim, or the aggressive behavior of the abuser. In this project I will analyze the feminist perspective of†¦show more content†¦Other women seek help within their family and friends but do not have the family support system need to help them. Many women feel alone and helpless because of the lack of knowledge of resources such as the National Domestic Violence Hotline available to assist them 24/7. Another reason why vic tims of domestic violence stay in their situation is fear of not being believed, and that they will be the one projected to have the problem. But for many women who are victims of domestic violence, the choice is to stay with the abuser and try to avoid being beaten, or to leave the situation and face a life of financial insecurity for her children and herself. Based on personal experience as a Military Police Officer I have unfortunately dealt with quiet of bit of cases which involved domestic violence. I believe that there are more domestic violence cases in the military then in the civilian world, due to the stress at work and the prolonged separation of family members. When I read about the fact that most domestic violence cases are not reported I thought of the fact that most times when domestic violence cases are being reported to authorities it is not by the victim or subject but a neighbor or witness. Most times when Military Police arrive on scene to a domestic violence situation it is instantly quelled due to the factShow MoreRelatedDomestic Violence : The United States Essay1405 Words   |  6 PagesEvery minute, twenty people are physically abused by an intimate partner in the United States. According to the Natural Coalition of Domestic Violence, after one year more than ten million men and women become a victim of domes tic violence. Every year someone new becomes a victim. Every year someone gets physically harmed or even death. Every year someone finds freedom from domestic violence. Domestic violence is an act which one individual purposely harms another, usually someone they are closeRead MoreDomestic Violence in the United States1516 Words   |  7 PagesDomestic violence affects a large amount of relationships in the United States each year. As the times have changed, abuse has become less accepted as a normal occurrence, and society has begun working together to provide awareness towards violence in intimate partner relationships. â€Å"Problems of family violence are potentially the most destructive in our society† (Kurland 23). Domestic violence is a problem that begins in the home, and spreads to affect the world around it. Violence is present inRead MoreDomestic Violence During The United States1174 Words   |  5 Pagesbeen recorded that approximately â€Å"2,000,000 injuries, and 1,300 deaths are caused each year as a result of domestic violence† (Centers for Disease Control and Prevention). This harm to and loss of life is due to the n egligence of all Americans, in regards to the monitoring and managing of domestic violence in the United States. In order to combat the blatant disregard for domestic violence victims, Americans must support, aid, and assist those who fall victim to this crime, as well as educate thoseRead MoreThe Effects Of Domestic Violence On The United States1634 Words   |  7 Pagesevaluate an aspect of my own culture. Additionally, I will also examine an aspect of another culture from an emic perspective, to better comprehend the behaviors, culture systems and beliefs. I will specifically be analyzing the topic of domestic violence in the United States, by examining the leading causes to this major issue, I will not only be able to better comprehend the magnitude of the affects this issue has in women but also the lasting consequences it has on today’s children. On the other handRead MoreThe Effects Of Domestic Violence On The United States1444 Words   |  6 PagesMillion of women in the United States are physically, and emotionally abuse by an intimate partner each year. Domestic violence is a situation that harms and kills most particularly women, children, and families members. As a result, battering of women is one of the foremost causes of injury to women. The growing awareness of how pervasive and destructive this situation is in our society, and the violence that accompanies it, has created a wide variety of programs, shelters, educational endeavorsRead MoreThe Effects Of Domestic Violence On The United States1443 Words   |  6 PagesMillions of women in the United States are physically, and emotionally abused by an intimate partner each year. Domestic violence is a situation that harms and kills most particularly women, children, and families members. As a result, battering of women is one of the foremost causes of injury to women. The growing awareness of how pervasive and destructive this situation is in our society, and the violence that ac companies it, has created a wide variety of programs, shelters, educational endeavorsRead MoreThe Effects Of Domestic Violence On The United States930 Words   |  4 Pagesthroughout the United States. Even though this abuse is evident in all states, some states, such as Kentucky, experiences higher rates of wife abuse than others. As I attempt to uncover an explanation for the high rates of wife abuse in Kentucky, I will discuss wife, or spousal, abuse in Kentucky and across the United States, the various programs offered to battered women in Kentucky, and how cell phones have both positive and negative effects for the victims of abuse. Although Domestic Violence is aRead MoreDomestic Violence During The United States830 Words   |  4 Pages Before reading the first two parts of Til Death Do Us Part I knew that domestic violence was a growing issue in the United states, but it was a surprise to find out that the state that we live in for more than fifteen has been place in the top ten nationally in the rate of women murder by men (Pardue, Smith, Hawes, Hauff, 2014). The first part gave the statics on how many victims were turned away in 2012-2013 from shelters around South Carolina which was a total of 380. The reason why thisRead MoreDomestic Violence : The United States Department Of Justice956 Words   |  4 PagesCurrently in the United States, every one-in-three women and one-in-four men are abused physically by their chosen partner in their lifetime. It is estimated that twenty people per minute are physically affected by their partner; therefore, there are around ten million people every year battered by their partners (â€Å"Statistics† National Coalition Against Domestic Violence). The United States Department of Justice defines domestic violence as: â€Å"We define domestic violence as a pattern of abusive behaviorRead MoreDomestic Violence : The United States Department Of Justice Essay1028 Words   |  5 PagesSonia Bola CRJ 101 Professor Watson 5 October 2016 Encountering Domestic Violence Stuart F. Delery, Former Acting Associate Attorney General, (2015) states The month of October is observed as Domestic Violence Awareness Month around the country by advocates, law enforcement, prosecutors, survivors and many others to raise public awareness about domestic violence. According to the United States Department of Justice (2016) domestic violence is defined as a pattern of abusive behavior in any relationship

Effects of Music on Teenagers free essay sample

Every teenager who listens to music interprets the lyrics in a different way. Some people take out a deeper meaning from the lyrics, even going to the point of saying that music saves their lives. Music holds a powerful effect on an individual because it can stimulate and provoke multiple responses-?physiological, emotional, cognitive, and behavioral. But, every genre of music gives its own message to teenagers. Some may enforce positive actions, while some condone negative actions.Rap has the negative status of objectifying women and promoting gang violence. On the other and, rock has a positive reputation because It helps people cope with their problems and Identify with their peers. Everyone. Teenagers Included. Is susceptible to internalizing the messages they hear over and over again. The degree of those messages determines the effect on the teenager. Every genre of music effects teenagers in a different ways. In some genres, like rock, the positive effects outrun the negative, and in some genres, like rap, the exact opposite happens. Alternatively, raps main focus is free styling and looping words to create a story in a flowing rhyme, keeping it in sync with the beat.In most cases, like the rapper Machine Gun Kelly, the artist performs by themselves with the assistance with a DC or MAC to play the musical tracks for the songs. Rap displays a larger emphasis on lyrics, while rock likes to have a variety of sounds. In the rock genre, its all about expression. Rock music can speak about all kinds of topics, from love to some not so pleasant. Many rock musicians sing about real issues that teens are going through, which helps teens find motivation. Adolescents sometimes find it difficult to communicate with older people, most of the time because of ideological differences.This often makes teens with problems shy away from seeking help and guidance. Teens find hope and encouragement when they listen to music that talks about problems they are going through. The identification they are experiencing with the rock song lyrics can also help teens to become more tolerant and open-minded. A study published for the 9th International Conference on Music Perception and Cognition reported that mood regulation is one of the most important reasons why people listen to their favorite music (Uncommon).Rock music can help mood regulation in teenagers, especially females who are prone to mood swings and depression. For example, the song Eye Of the Tiger by Survivor can cause teens to associate with the underdog. The song provides a positive message, while keeping an uplifting tone throughout the whole song. The music provides depressed teens with a mode to forget their problems and participate in healthy activities, such as dancing. While rock has been given a positive reputation for allowing teenagers to express themselves, some have given it a poor reputation because it can intensify emotions.While those people who are naturally happy and cheerful are less likely to have effects from the negative lyrics, other, less fortunate teens might not be the same. Adolescents who are susceptible to negative, violent, or depressive thoughts are more likely to have those feelings, and act on them, after listening to rock sic. At the same time, if a person is not experiencing those negative thoughts, the music can act as a catalyst, causing the teen to have these thoughts and urges. The rap genre is often given a negative reputation for the way it effects teenagers.Critics of the genre say that the music objectifies women and promotes drug use. For example, the song 9 Problems by Jay-Z, repeatedly refers to a woman as a pitch. This song allowed women to be categorized under the vulgar name pitch, because of the high reception the song received in the media (The Portrayal of Women Within Popular Music). A cent study by WebMD reports that teenagers who listen to rap music are more likely to have babies and engage in risky sexual behavior (Cheerier). Apparently, this behavior is because the lyrics in many rap songs promote the idea of women as nothing more than sexual objects.Correspondingly, it was shown that youth who listened to rap songs were more likely to get involved with drugs. This is because in some songs, the artists refer to drugs that the person has not heard of, which sparks their curiosity to learn and most likely try the new drugs. The rap genre can also give teenagers a false sense of longing. When they hear about, or see in the videos, expensive possessions, they feel that if they spend their money to buy that stuff, they can be just like the people in the videos.The media then uses this to their advantage to market products to the younger, naive generations. Even though rap is seen as a vulgar music genre by a large population, it can be positive for teenagers. It makes a person feel good about who they are because even if they may not live a good life, they can relate to the lyrics in a particular song. This is different than the false sense of belonging because instead of the individual trying to fit in, they are finding the place where they fit in, while not changing themselves. Rap lyrics also help youth feel free and powerful.They are free to express what they want to express. Listeners of the music have even gone to the extent of calling the rap genre a religion for troubled youth because it provides them with an outlet to express themselves and something to believe in. It is evident that rock and rap are very different genres that expose different messages to teenagers. But, no matter the genre of music, studies have shown that the use of music videos how an increased open-mindedness to deviant behavior among teenagers. The music videos reinforce what is being heard in the lyrics with the use of visuals.They usually exhibit sexual innuendo, acts of aggression, and gender stereotypes. Research has shown that, after watching videos, men said it was necessary to pursue the women to an inappropriate extent, while women thought it was annoying and/or disturbing. Men scored higher in relations to attitudes for sexual overtones, while women scored higher on the acceptance of interpersonal violence. The results of the study also showed that women ho were often subjected to violent music videos were more accepting of violence.